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The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.，这一点在雷电模拟器官方版本下载中也有详细论述
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再比如工厂中的流水线作业，对机器人的可靠性需求也会上升一个量级，手术机器人是对可靠性要求最高的应用场景之一。